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bulk RNA-seq(3):Trim the read files using trim_galore
Tip We will install Trim Galore and trim the merged read files to improve the quality of our data.
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bulk RNA-seq(2):Quality Control with FastQC and MultiQC
FastQC for Quality Checks FastQC provides a simple way to perform quality control checks on raw sequence data.
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bulk RNA-seq(1):Concatenation of raw read files
Concatenation of Raw Read Files To begin with, my operating environment is Linux Ubuntu 20.
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What is the RNA-seq analysis?
Overall description of RNA-Seq The transcriptome has a high degree of complexity including multiple types of coding and non-coding RNA species, like mRNA, pre-mRNA, microRNA, and long ncRNA.
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