Bulk RNA-seq (9): Gene Ontology Analysis of Differential Expression
Note Next, we will perform Gene Ontology (GO) to unearth the biological processes, cellular components, and molecular functions our significant genes are involved in.
Read MoreBulk RNA-seq (8): Differential Analysis and Volcano Plotting with DESeq2
Note Here, we demonstrate how to sift through the complex data to identify genes of interest and showcase their expression patterns through an elegant volcano plot.
Read MoreBulk RNA-seq (7): From ENSEMBL ID Cleanup to Gene Name Conversion
Note In the realm of RNA-seq analysis, one often encounters the challenge of working with ENSEMBL IDs that include decimal points, a result of gene versioning in databases.
Read MoreBulk RNA-seq (6): Normalization and PCA Visualization in RNA-seq Data Analysis with DESeq2
Note In this blog post, we’ll dive into a practical guide on using DESeq2 for differential expression analysis in RNA-seq data.
Read MoreBulk RNA-seq (5): Streamlining Mapping with a Custom Linux Script
Note To overcome the inconvenience of manually mapping each RNA-seq sample to the reference genome, I’ve developed a Linux shell script.
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